Fluorescent Light Microscope – FLUO Series

The Fluorescent Light Microscopes from Optika Microscopes offer advanced imaging capabilities using fluorescence and phosphorescence to examine both organic and inorganic materials. Available from Apex Scientific in South Africa, this series includes Epi Fluorescence microscopes with specialized illuminators for precise incident light control.

These microscopes are ideal for highlighting specific features within specimens, making them essential for detailed biological and material science research. Explore our range of laboratory equipment or visit our Insights page for helpful articles and expert advice.

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Flourescent Microscope

The Fluorescent Light Microscopes from Optika Microscopes offer advanced imaging solutions for scientists across South Africa. Available from Apex Scientific, these microscopes are ideal for visualizing biological and chemical specimens using fluorescence. They highlight fine structural details by converting short-wavelength excitation light into longer-wavelength emission, creating vivid and precise images.

 

Principle of Operation

Fluorescent Light Microscopes work by exciting fluorophores within a sample using light of a specific wavelength. The emitted fluorescence, which is of a longer wavelength, is isolated from the excitation light using an emission filter. Core components include a light source (such as a mercury-vapor lamp or high-power LED), an excitation filter, a dichroic mirror, and an emission filter. These elements must be precisely matched to the fluorophore in use. Typically, one fluorophore is viewed at a time, while multi-color imaging is created by merging separate channels.

 

Epifluorescence Microscopy

The FLUO Series relies on epifluorescence microscopy—a widely used method in life science research. This system uses the same objective lens for both excitation and emission. As a result, the signal-to-noise ratio is enhanced, producing sharper images. A dichroic beamsplitter ensures that only the emitted light reaches the eyepiece or camera while reflecting back any excess excitation light.

 

Ideal for Malaria and TBC Screening

The B-383LD1 LED Fluorescent Microscope is especially useful for fast diagnosis of malaria and tuberculosis. It uses the acridine-orange staining technique to deliver reliable results. For enhanced performance, we recommend the optional W-PLAN 50x/0.75 no cover glass objective. This setup balances high image quality with cost efficiency.

 

Versatile Observation Techniques

Beyond fluorescence, these microscopes support other observation methods, including brightfield, darkfield, and phase contrast. This flexibility allows researchers to explore various sample characteristics using a single instrument.

Accessories

microscope slide
M-005 - Micrometric slide, 26x76mm. Provides precise measurement capabilities, ensuring accurate calibration and enhanced detail analysis under microscopes.
Microscope cleaning kit
15104 - Cleaning kit. Ensures optimal lens clarity and performance by safely removing dust, smudges, and debris from optics.
Microscope Dust Cover
DC-002 - Plastic dust cover. Protects the microscope from dust and contaminants, ensuring long-term durability and optimal performance.
microscope solar charger
M-069: Solar charger: Provides eco-friendly power supply, ensuring continuous microscope operation with reliable solar energy charging.
microscope mirror
M-971: Plane-concave mirror, with base for B-190/290 Series. Provides an alternative light source, ensuring reliable illumination for clear and detailed microscopy observations.
Microscope Polarizing set
M-174: Polarizing set (filters only) for B-190 & B-290 series. Enhances contrast and reduces glare by filtering polarized light for detailed and precise microscopy observations.
Microscope Immersion oil
15008: Immersion oil, 10ml. Enhances image clarity and resolution by reducing light refraction for high-magnification microscopy observations.
Microscope Rotating Table
M-175: Rotating table for polarising set (for models with 150x139mm rackless stage). Enables smooth 360° rotation for precise polarization analysis, ensuring accurate sample positioning and observation.
Microscope
M-185: Darkfield condenser for dry objectives. Enhances contrast by directing oblique light, making transparent specimens more visible under darkfield illumination.
Microscope Projection Lens
M-118: 0.75x C-Mount projection lens. Reduces magnification while maintaining image clarity, providing a balanced field of view for digital imaging.
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For expert advice and more information, read our article, Choosing the Right Microscope for Your Laboratory: A Detailed Guide. You can also explore our Insights page for technical resources and browse our full product range to find the best solution for your application.

B-290LD – LED Fluorescence Microscopes

B-292LD1.50Fluorescence binocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.
B-293LD1.50 Fluorescence trinocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.
B-292LD1Fluorescence binocular microscopes especially designed for tubercolosis and malaria analysis IOS W-PLAN 100x/0.80 (No Cover, Dry), with anti-fungus treatment.
B-293LD1 Fluorescence trinocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.

 

B-383LD – LED Fluorescence Microscope

B-383LD Entry-level laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives. The extremely powerful LED fluorescence illuminator is combined with blue excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

 

B-383FL – HBO Fluorescence Microscope

B-383LD Laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives. The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

 

B-510LD – LED Fluorescence Microscope

B-510LD4 Advanced routine fluorescence microscope for transmitted brightfield and fluorescence observations with IOS W-PLAN objectives. The extremely powerful LED Fluorescence Illuminators are combined with corresponding excitation filter sets for the visualization of most fluorochromes. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.
B-510FLAdvanced routine laboratory microscope for brightfield and fluorescence observations with Semi-Apo IOS W-PLAN F objectives to enhance the visibility of the sample and increase the overall contrast. The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

 

B-1000FL-LED – LED Fluorescence Microscope

B-1000LD4 The modular OPTIKA B-1000 can stand a LED fluorescence attachment, helping you working in a comfortable way during extended periods of use and performing reliable, accurate and rapid diagnosis benefiting from modularity, which gives the chance to create customized configurations tailored on customer needs. Versatile, robust, durable and sturdy, B-1000 offers premium quality optics (including Apo and Semi-Apo objectives), the state-of-the-art, exclusive X-LED8 (8 W) illumination system, designed by OPTIKA and the Koehler diaphragm. B-1000 gives multiple options as manual or motorized configuration, with a variety of objectives, stages and condensers.

 

B-1000FL-HBO – HBO Fluorescence Microscope

B-1000FL-HBO– The modular OPTIKA B-1000 can stand a HBO fluorescence attachment, helping you working in a comfortable way during extended periods of use and performing reliable, accurate and rapid diagnosis benefiting from modularity, which gives the chance to create customized configurations tailored on customer needs. Versatile, robust, durable and sturdy, B-1000 offers premium quality optics (including Semi-Apo objectives), the state-of-the-art, exclusive X-LED8 (8 W) illumination system, designed by OPTIKA and the Koehler diaphragm. B-1000 gives multiple options as manual or motorized configuration, with a variety of objectives, stages and condensers.

 

IM-300LD – LED Fluorescence Microscope

IM-300LD2Routine inverted fluorescence microscope for transmitted brightfield, phase contrast and fluorescence observations with IOS LWD W-PLAN PH objectives. The LED fluorescence illuminators are combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green). LED fluorescence ensures unparalleled convenience eliminating warm-up/cooldown times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and highquality specimen view.
IM-300LD4 Advanced fluorescence inverted microscope for transmitted brightfield and fluorescence observations with IOS U-PLAN objectives. The extremely powerful LED Fluorescence Illuminators are combined with corresponding excitation filter sets for the visualization of most fluorochromes. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.
IM-300LD4DAdvanced fluorescence inverted microscope for transmitted brightfield and fluorescence observations with IOS U-PLAN objectives. The extremely powerful LED Fluorescence Illuminators are combined with corresponding excitation filter sets for the visualization of most fluorochromes. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view. This model is equipped with an Intel micro-PC, 15.6” touch screen 4K, a 6Mpx high-sensitivity color camera, Optika ProView image analysis software for fluorescence and wireless mouse and keyboard.

 

IM-300F – HBO Fluorescence Microscope

IM-300FRoutine inverted fluorescence microscope for transmitted brightfield, phase contrast and fluorescence observations with IOS LWD W-PLAN objectives. The HBO fluorescence illuminator is combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green). Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.
IM-300FL4Advanced inverted microscope for brightfield and fluorescence observations with Semi-Apo IOS LWD U-PLAN F objectives to enhance the visibility of the sample and increase the overall contrast. The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.

 

IM-5F – HBO Fluorescence Microscope

IM-5FLDOPTIKA IM-5FLD is designed to be the ultimate inverted research fluorescence microscope, combining innovative technology with unparalleled comfort. You can achieve astonishing images thanks to the IM-5FLD superb optics, Köhler condenser and the pioneering motorized selection of the more suitable LED source, automatically chosen in accordance with the selected fluorescence filter set (B, G, UV, plus optional ones). Last but not least, IM-5FLD boasts the highest F.O.V. available on an inverted microscope. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.

Insights Article: Choosing the Right Microscope for Your Laboratory: A Detailed Guide

Product Enquiry Handbook: Stereomicroscopes

Product Enquiry Handbook: Light Microscopes

Operating Manual

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